Dear Dr.Wang, When I transformed the TevCas9 vector into the...

Protocol: Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor

Dear Dr.Wang, When I transformed the TevCas9 vector into the Phaeodactylum tricornutum, I found there were multiple cuts of the target gene of the single clone grown on the zeocin plate . I only design the gRNA of Cas9 and ignore the TEV site. I used gene gunning to bombard the vector Read more Read less

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