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For carrying out the germination assay for Aluminium stress...

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POONAM CHETRY Sep 4, 2019
protocol Protocol: Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach
For carrying out the germination assay for Aluminium stress in the wheat and rice genotypes what ratio of CaCl2 + AlCl3 be appropiate if 0.5mM of CaCl2 and 100 microM of AlCl3 is chosen
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Bedabrata Saha Author Answered Sep 9, 2019

Newe Ya'ar Reseach Centre, Agricultural Research Organization, Ramat Yishay Ramat Yishay

Poonam you can keep the CaCl2 dose constant at 0.5mM and play with the AlCl3 dose. CaCl2 treatment is basically given to enhance the uptake capacity of Al3+. There are also many reports where CaCl2 has not been used, still plants are showing aluminium toxicity symptoms. Its totally upto you on how you will conduct your experiment.
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