Hi, may I ask for which solutions the Bradford assay can be...

TB
Theea Becirevic
Aug 22, 2019
protocol Protocol: Bradford Protein Assay
Hi,
may I ask for which solutions the Bradford assay can be used, is it suitable for to measure a protein conc. from organic cream?
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MICHAEL D SMALLING Answered Aug 23, 2019

Louisiana Tech University

It also depends on what your "organic cream" is. If it contains a lot of non-protein material, (i.e
Lipids) you may need to extract protein content before assaying using something like trichloroacetic acid to precipitate proteins.

Mike
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MICHAEL D SMALLING Answered Aug 23, 2019

Louisiana Tech University

A Bradford's assay is the most common assay for protein concentration. If protein concentration for your sample is completely unknown, you may need to dilute it sufficiently to bring it into the range of your prepared standards. Avoid using SDS or ionic detergents in sample preparation as they may interfere with results.

Mike
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