Hi deer, may I ask you why we using TFA at the last step?

protocol Protocol: Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
Hi deer, may I ask you why we using TFA at the last step?
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Harald Keller Author Answered Aug 22, 2019

INRA, Université Côte d’Azur, CNRS, Institut Sophia Agrobiotech, France, France,

Dear Ihab Nazar Al-Abasi
TFA is used to acidify the solution and protonate the carboxyl group of SA (and other organic acids), thereby discharging the group and making the molecules accessible for reverse phase chromatography.
Best regards
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