Hi, I'd like to ask some questions about polysomes. Specifically...

I assume you have labeled the peaks correctly, although we have not specifically tested to be sure those peaks represent the 40S, 60S and 80S. We also have not calculated changes in these subunits, although I would think you could calculate the area under the curve for each peak and represent as a ratio or percentage of the total area under the curve to account for any differences in sample loading. To me it looks as though your KO samples may be slightly underloaded compared to the WT samples as all the peaks seem to be diminished compared to the WT.

同学你好，我是安徽医科大学的学生，想请问一下您测吸光度的仪器是什么，如何做出曲线的呢，希望您方便时能给予解惑，十分感谢