Dear Julia, Thanks for your detailed steps. Here are some questions...

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shen lihang
Jul 27, 2019
protocol Protocol: Human, Bacterial and Fungal Amplicon Collection and Processing for Sequencing
Dear Julia,
Thanks for your detailed steps. Here are some questions confused me.

1.Does "yeast cell lysis buffer" in step A1 refer to the TE Buffer or yeast cell lysis solution in MasterPure Kit?
2.Is it correct that after we have lysed bacteria and yeast by Readlys
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Julia Oh Author Answered Jul 29, 2019

National Human Genome Research Institute, National Institutes of Health, MD

The yeast cell lysis buffer is the yeast cell lysis solution in the MasterPure kit, yes. And yes, then there is no need to use the lysis buffer from the PureLink kit.
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