Hi,While storing the OMVs at -80 °C, did you use any glycerol...

OMVs stored at –80˚C prior to proteomic analysis were stored as a pellet (not resuspended).

There is always a chance of degradation, however, we found the OMV preps very stable in these conditions. Nevertheless, we will not use OMVs stored for more than 2 weeks for experiments, although we have seen that they remain active even after 3-4 weeks in 4C.

Hi,

Thanks for the quick revert to my question.

I have another concern about storing the OMVs in PBS at 4C. How many days can they stored in such condition, will there be any chance of degradation to OMV activity upon long term storage in PBS at 4C?

We store OMV pellets (not resuspended in any liquid) in -80C before using it for LC-MS/MS analysis to deeming the proteome of OMVs. We do not use -80C storage of OMVs if we are planning to conduct experiments with the OMV preparation.
Otherwise, for normal storage, we store OMV resuspended in PBS buffer at 4C.