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Hello Dr. Gillotin,I wanted to confirm the E3 buffer contains...

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V Cerv Jul 9, 2019
protocol Protocol: Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Hello Dr. Gillotin,

I wanted to confirm the E3 buffer contains 500mM Tris-HCl as the other buffers require less concentrated Tris-HCl.

Thank you for your time!
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Sébastien Gillotin Author Answered Aug 7, 2019

Discovery Research, Neuroscience, MSD

Hello,

Yes the concentration is higher for E3 than it is for E2. Also note the difference in pH.

Best of luck with your research.
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