Hi! I am a PhD student using NGM. When I add the cholesterol...

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Tania C S
Jul 4, 2019
protocol Protocol: Common Worm Media and Buffers
Hi! I am a PhD student using NGM. When I add the cholesterol to NGM .Which temperature NGM has to be?Cholesterol always precipitates and forms white particles and I am not sure if this is a problem of homogenization when pouring the plates and if it can impede my C.elegans worms to develop Read more Read less more less
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jyothi kotha Answered Aug 6, 2019

srm university

after adding cholesterol to ethanol place it on vertex shaker for 15 mnts then it will dissolve and add chol. to ngm at 55degress
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