I would also like to ask you if you add OPT in your blank samples!...

protocol Protocol: Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate
I would also like to ask you if you add OPT in your blank samples! I noticed that in the protocol of HIlf and Hissin for gssg/gsh in tissue homogenates they don’t use OPT in blank samples they just add MeOH , which is the solvent of OPT. Are you doing the same in your blanks ?
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Vikas Singh Author Answered Jun 21, 2019

Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India

In our protocol we have used OPT solution in blank without cell lysate.
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