Hello, while reading the protocol i noticed that in the recipes...

protocol Protocol: Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate
Hello, while reading the protocol i noticed that in the recipes you provide the way to create OPT at a concentration of 10mg/ml while in the procedure you use OPT at a concetration 0f 1mg/ml. Which one is the one that you actually use in the experiment ?
Also, in the recipes you prov
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Vikas Singh Author Answered Jun 9, 2019

Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India

Dear Marilena,
For the first query, we prepared a stock solution of 10mg/ml OPT and added in such a way that final concentration of OPT in reaction mixture becomes 1 mg/ml.

For the second query, I apologize for error in recipe section. We prepared a stock solution of 4 M n-ethyl maleimide (NEM) instead of 0.4 M and finally added NEM with sample from 4 M stock in such a way that final concentration of NEM is 0.04 M.
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