Improve Research Reproducibility A Bio-protocol resource

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sangeetha vijay May 23, 2019
protocol Protocol: Isolation of Human PBMCs
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I have kept mononuclear cells( separated from peripheral blood using ficoll ) in RNA later( 250 microlitre)+DEPC-PBS( 50microlitre) at 4 degree Celsius overnight and removed the RNA later the next day, by centrifugation and pre Read more Read less more less
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Santosh K Panda Author Answered Jun 10, 2019

Infectious Disease Biology, Institute of Life Sciences, India

You could freeze without removing RNAlater
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