Hello, I have a question concerning step B14. How do you solubilize...

protocol Protocol: Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
Hello, I have a question concerning step B14. How do you solubilize the dry residue? I have great trouble with SA recovery after spiking and I believe this step is crucial. I cannot solubilize the dry residue by vortexing...
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Valérie Allasia Author Answered May 23, 2019

INRA

Hello,
if you have respected the starting proportion between the quantity of fresh materail and the volume of solvent at the beggining of extraction (2 extractions of the ground material) normally the quantity of residues obtained (of green color) after evaporation of the solvents, and speed vac less than 30 min must recover in the acidic MeOH. Try to add first 10 μL of 100% MeOH on the residues and then add 90 μL H2O containing 0.1% TFA, vortex 1 min.
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