Hi,Can this protocol be worked out on whole blood samples directly...

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Neha Rana
May 21, 2019
protocol Protocol: Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry
Hi,
Can this protocol be worked out on whole blood samples directly fixed with protein stabilizer and stored at -80C ?

Thank you.
Neha
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Holden T. Maecker Author Answered May 24, 2019

Immunity, Transplantation & Infection, School of Medicine, Stanford University, Stanford

No, that’s not possible once the cells are in Proteomics Stabilizer, as it contains a fixative.
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Neha Rana Answered May 23, 2019

University of Bergen

Thank you very much for the reply . Yes , I understand that , But I actually meant if cytokinins stimulation be done in freezed samples . That doesn’t looks like a possibility though !
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Holden T. Maecker Author Answered May 23, 2019

Immunity, Transplantation & Infection, School of Medicine, Stanford University, Stanford

Yes, it's absolutely fine to skip the resting/stimulation, put blood directly in Proteomic Stabilizer, and proceed from there. This allows for ex vivo phenotyping + assessment of basal signaling states.
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