Hello,I have a question regarding plating the organoids in the...

HV
Himani Vaidya
Apr 3, 2019
protocol Protocol: Immunofluorescent Staining of Mouse Intestinal Stem Cells
Hello,

I have a question regarding plating the organoids in the 8 well chamber. Do I need to put the matrigel with organoids in a dome shape or plate it flat with the base of the well. I tried using your protocol but it seems that the organoids in the middle of the dome did not
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