Improve Research Reproducibility A Bio-protocol resource

I Dissolved 0.1 g of Coomassie Brilliant Blue G-250 in 50 ml...

ha
habib afridi Apr 1, 2019
protocol Protocol: Bradford Protein Assay
I Dissolved 0.1 g of Coomassie Brilliant Blue G-250 in 50 ml of methanol 95% and added 100 ml 85% (w/v) phosphoric acid (H3PO4), diluted up to 1 liter using distilled water. My question is that when I added water then the colour of comassie brilliant blue G-250 changed from brown to blu Read more Read less more less
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

MICHAEL D SMALLING Answered Apr 2, 2019

Louisiana Tech University

Habib, it sounds like your DI water may be acidic.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer