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Hello,In this protocol, it states that for lentiviral production,...

Farrah Aram Mar 21, 2019

Protocol: Qualitative in vivo Bioluminescence Imaging

Hello,

In this protocol, it states that for lentiviral production, the HEK293T cells are transfected using FuGENE 6 and the FUGLW plasmid. May you clarify at which step the packaging and envelope vectors are added?

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Farrah Aram Answered Mar 31, 2019

None

Hello,

Thank you for your reply to my previous question. I do, however, have an another one. Can you suggest any commercially available alternatives to the FULGW plasmid used in this protocol? Thank you in advance.

Sincerely,
Farrah

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Pritinder Kaur Author Answered Mar 24, 2019

School of Pharmacy & Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Australia, Australia,

Hi Farrah

Apologies for not making this clear in the protocol. The HEK293 cells are co-transfected with FUGLW AND the packaging vectors to generate replication-defective lentivirus. We cited Sinha et al., 2016 which explains this methodology a bit better.

Sincerely
Pritinder kaur

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