Dear Petros,Thank you very much for your explanation.One more...

GF
Giacomo Foschi
Mar 15, 2019
protocol Protocol: Culture of Megakaryocytes from Human Peripheral Blood Mononuclear Cells
Dear Petros,

Thank you very much for your explanation.
One more question:
you suggested to manipulate cells as less as possible after 3/4 days. Therefore, in order to start phase 2 or phase 3, It is better to add phase 2/3 medium to old medium without pelleting cells,
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