Dear authors, I am trying to microinject cells into the perivitelline...

protocol Protocol: Zebrafish Embryo Xenograft and Metastasis Assay
Dear authors,
I am trying to microinject cells into the perivitelline space but I found it very difficult. I have a very small quantity of well injected embryos at the end of the experiment because I don´t know how to approach this site. Have you got any recommendation? Thank you ver
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Ilkka Paatero Author Answered Mar 5, 2019

Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Finland, Finland,

Dear Patricia,

I have had also some troubles when transplanting into perivitelline space, and in most experiments I use other anatomical sites. For me it works best, if I inject into perivitelline space from posterior side and proximal to the body. So, approach the perivitelline space from tail side of the embryo, quite close to body and try not to extend the needle too deep in as it goes easily inside yolk. Having the needle coming in at a lower angle can also help. I wish that you find these tips useful.

best,
Ilkka
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