Whether I should use the Spectra/Por® 1 Dialysis Membrane for...

AM
Akhil M
Mar 5, 2019
protocol Protocol: Trehalase Activity in Arabidopsis thaliana Optimized for 96-well Plates
Whether I should use the Spectra/Por® 1 Dialysis Membrane for the dialysis or is it about the pore size? Whether can I use pore size between 12000- 2400 MWCO for the dialysis?
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Hilde Van Houtte Answered Mar 6, 2019

SESVanderHave

A membrane with a pore size between 12- 24 kD MWCO should also retain the Arabidopsis trehalase (64 kD). Note: In some cases, the dialysis step can be omitted, for instance when using plant material with low amounts of carbohydrates and ions.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer