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To whom it may concern,Good morning, I'm writing because I'm...
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Paolo Motta Feb 25, 2019
To whom it may concern,
Good morning, I'm writing because I'm interested in trying your protocol (I need to resolve a 4 kDa protein), but I would like to ask a clarification.
In your Protein Sample Buffer recipe you add 400 mM EDTA, but EDTA solubility in water should be much low Read more
Good morning, I'm writing because I'm interested in trying your protocol (I need to resolve a 4 kDa protein), but I would like to ask a clarification.
In your Protein Sample Buffer recipe you add 400 mM EDTA, but EDTA solubility in water should be much low Read more
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César Enrique Rivera Author Answered Feb 25, 2019
Energy and Enviroment, IDEA, Venezuela
Good afternoon, we appreciate your interest in our protocol. In the sample buffer the EDTA was used since was the reagent available at the moment, but it can be changed for DTT at the same concentration or β-Mercaptoetanol. EDTA is not necesarry for a correct run, and we did not used reducing agent in the Sample Buffer nor boil the samples since we needed the native proteins. Best Regards.
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