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Hi Pooja,My borage seeds have thick and black seed coats that...
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Lee Chen Feb 21, 2019
Hi Pooja,
My borage seeds have thick and black seed coats that make the staining solution hard to get in contact with the endosperm or other tissues inside the seeds. What do you think can be done to improve the permeability of the staining solution through a thick seed coat? Read more 
My borage seeds have thick and black seed coats that make the staining solution hard to get in contact with the endosperm or other tissues inside the seeds. What do you think can be done to improve the permeability of the staining solution through a thick seed coat?
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Michael Onyedika Eze Answered Oct 21, 2019
University of California Davis
An option is to bisect the seeds longitudinally through the centre of the embryo. In that case, use 0.25% to 0.5% TTC instead of 1% TTC solution.
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