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Dear Dr. La torre. sorry aganin but i have another question...
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Ivana Barravecchia Jan 29, 2019
Dear Dr. La torre. sorry aganin but i have another question on this passage of protocol:
"From Day 7 medium should be changed every other day".
When you change the medium, you change all or half? there is the risk when you remove the medium to take away also organoids? How can Read more
"From Day 7 medium should be changed every other day".
When you change the medium, you change all or half? there is the risk when you remove the medium to take away also organoids? How can Read more
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Anna La Torre Author Answered Jan 29, 2019
University of California
Hi again! You can change only half the medium. After day 15 or so, the EBs are bigger and then, if you notice that the medium gets acidic quickly, you can do a full media change. The easiest way is to tilt the plate so all the EBs move to the bottom of one side and slowly (don't use suction! only a pipette) remove the media, add fresh media and you are done. Auguri!
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