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Hi, I have isolated bone marrow stem cells for mast cell isolation....

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Swati D Sonkawade Jan 25, 2019
protocol Protocol: Isolation and Culture of Bone Marrow-derived Mast Cells
Hi,
I have isolated bone marrow stem cells for mast cell isolation. I was wondering if I can use adherent cells from the same culture for the macrophage differentiation?
Thank you for your feedback,
Swati
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Krisztina V. Vukman Author Answered Jan 25, 2019

Dublin City University

Hi, After the peritoneal lavage if you leave peritoneal cells in medium (withouth WEHI-3 condotioned medium) for few hours, macrophage will adhere and you can use them. I think it should work from the mast cell culture as well.
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