Hi, what brand cell lifter did you use. When we use it, the...

yy
yalong yang
Jan 24, 2019
protocol Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Hi, what brand cell lifter did you use. When we use it, the cell is damaged seriously.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Ran Chen Author Answered Jan 23, 2019

Zhejiang JFK Biological Technology Co. Ltd.

Sigma.
Macrophages could attach to the dish very tightly, you'd better to add Trypsin-EDTA to the dish and incubate for 2 min to loose the macrophages before using cell lifters.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer