Dear De La Torre, I started to apply its exhaustive protocol yestarday (dir the forse time). I used R1 cell Line ti generete EBs. My questions concern on the transfer of the EBs from from 96 dell to 6 well. how many EBs can i transfer in each well? What could I use to transfer ebs? pasteRead moreRead less
Hi! We normally use a pipet with a 1000 tip. A pasteur will work too since it's big enough so the EBs are not broken during the transfer. From a 96 well plate, we move them to 3-4 wells of a 6 well plate. Therefore, we plate about 24-30 EBs per well but you don't need to count, just distribute them more or less evenly. Good luck with your cells! PS: I really want to learn Italian!