Dear De La Torre, I started to apply its exhaustive protocol...

protocol Protocol: Retinal Differentiation of Mouse Embryonic Stem Cells
Dear De La Torre, I started to apply its exhaustive protocol yestarday (dir the forse time). I used R1 cell Line ti generete EBs. My questions concern on the transfer of the EBs from from 96 dell to 6 well. how many EBs can i transfer in each well? What could I use to transfer ebs? paste Read more Read less more less
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Ivana Barravecchia Answered Jan 25, 2019

Università di Pisa and Scuola Superiore Sant'Anna di Pisa

Dear Dr. La Torre, thanks so much for your feedback!
Today is D2 of EBs and everything seems ok!
Fingers crossed!

P.S. we could share our knowledge: I in Italian and you in retinal differentiation :) !
Thanks again and have a good weekend.
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Anna La Torre Author Answered Jan 23, 2019

University of California

Hi! We normally use a pipet with a 1000 tip. A pasteur will work too since it's big enough so the EBs are not broken during the transfer. From a 96 well plate, we move them to 3-4 wells of a 6 well plate. Therefore, we plate about 24-30 EBs per well but you don't need to count, just distribute them more or less evenly. Good luck with your cells!
PS: I really want to learn Italian!
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