dear fang: can i replace step3 with a low centrifugation step?...

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Shaobo Zhu
Jan 2, 2019
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
dear fang: can i replace step3 with a low centrifugation step? like 100g,10min? best shaobo
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Shaobo Zhu Answered Jan 21, 2019

Center for Plant Stress Biology Chinese Academy of Science

谢谢!
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Fang Xu Author Answered Jan 2, 2019

Shandong University

Hi Shaobo, I think you can add an additional step by centrifuge at 100g for 5min. It can further get rid of large particles. But I always have the filtering step.
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