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I am trying to adopt this protocol to Klebsiella and Staphylococcus...

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Alex Sem Dec 18, 2018
protocol Protocol: Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli
I am trying to adopt this protocol to Klebsiella and Staphylococcus bacteria, it needs some modifications but seem to me should work.
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