Slice did not stick to membrane ?

protocol Protocol: Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
Slice did not stick to membrane ?
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Jillian M. Daniel Author Answered Dec 20, 2018

School of Biomedical Sciences, University of Leeds, United Kingdom

Sorry to hear you have had trouble with the slices not adhering to the membrane. The most likely reason for this is that all the media has not been removed after transferring the slices on to the membrane. Other occasion when I have trouble with the slices adhering to the filter is when the tissue is not viable, may be due to it taking a bit to long to complete the prep or bacterial contamination.
I hope this is helpful
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