Hello Stéphanie, does this need to inoculate two leaves in...

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andy zhao
Dec 7, 2018
protocol Protocol: Bean Pod Mottle Virus (BPMV) Viral Inoculation Procedure in Common Bean (Phaseolus vulgaris L.)
Hello Stéphanie, does this need to inoculate two leaves in one plant? If I had inoculated one leaf in one plant , what's wrong will happen?
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andy zhao Answered Dec 8, 2018

Chinese Academy of Agricultural Sciences

Hello Stéphanie, thank you very much! I hope one day to have the opportunity to learn more from you and communicate with you!!
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Stéphanie Pflieger Author Answered Dec 7, 2018

The National Center for Scientific Research

We always inoculate one primary leaf of one plant.
We have tested inoculation of the two primary leaves but this does not improve the infection rate.
Good luck with your experiments and don't hesitate to ask me any other questions if needed.
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