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Dear authors,I would kindly like to ask you the weight percentage...
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Nathalie Agudelo Dec 2, 2018
Dear authors,
I would kindly like to ask you the weight percentage to prepare the SeeDB2G mounting solution from Histodenz powder (Omnipaque 350 solution). According to your previous publication, it should be 56.2% w/w iohexol in Tris-EDTA buffer, however, preparing the solution as 10 Read more
I would kindly like to ask you the weight percentage to prepare the SeeDB2G mounting solution from Histodenz powder (Omnipaque 350 solution). According to your previous publication, it should be 56.2% w/w iohexol in Tris-EDTA buffer, however, preparing the solution as 10 Read more
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Takeshi Imai Author Answered Dec 6, 2018
Kyushu University Fukuoka
To prepare SeeDB2G, dissolve 10g Histodenz in 7.5 mL Tris-EDTA (~57.1% w/w) as described in Recipes. The refractive index will be exactly the same as that of Omnipaque350 (1.4662).
In most applications, there will be no need for further refinement. Please note, however, that the immersion liquid for some glycerol lenses may have a slightly lower refractive index. For example, 92% w/w Omnipaque350 matches Type G immersion liquid.
As for Triton X-100, we recommend 0.1-0.5% to minimize morphological damages. 2% is too high for Triton X-100.
To further minimize morphological damages, we also suggest more gradual clearing with intermediate concentration steps.
In most applications, there will be no need for further refinement. Please note, however, that the immersion liquid for some glycerol lenses may have a slightly lower refractive index. For example, 92% w/w Omnipaque350 matches Type G immersion liquid.
As for Triton X-100, we recommend 0.1-0.5% to minimize morphological damages. 2% is too high for Triton X-100.
To further minimize morphological damages, we also suggest more gradual clearing with intermediate concentration steps.
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