Yes, it happen sometime. Make sure your tissus are well perfuse...

Protocol: Fluoro-Jade B Staining for Neuronal Cell Death

Yes, it happen sometime. Make sure your tissus are well perfuse with PFa4%-borax and cut at 25um max. If your tissus is too tick you will have higher background. You can also decrease a little the incubation time in the potassium permanganate (2-3min instead of 5).

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