We sought this protocol to learn how to do intradermal inoculation...

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Brian Weinrick
Nov 30, 2018
protocol Protocol: Preparation of Mosquito Salivary Gland Extract and Intradermal Inoculation of Mice
We sought this protocol to learn how to do intradermal inoculation of the mouse ear, not mosquito salivary gland dissection. The protocol is very clear and detailed but the technique is challenging, so we were unable to reproducibly deliver 20ul to the ear dermis. I think we could learn th Read more Read less more less
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Michael A. Schmid Author Answered Dec 2, 2018

University of Leuven

I completely agree that the intradermal inoculation is challenging and requires regular practice. A key challenge I found was that the ear may have several punctures after a few attempts, leading to leakage of the inoculated solution. Decreasing the injection volume to 5-10 µl may help.
A colleague follows a similar protocol by attaching the ear skin to a double-sided, sticky tape for the injection (not requiring forceps). Despite potentially being easier, the subsequent "tape stripping" may induce dendritic cell migration, which was a key readout for us.
Alternatively, one could inoculate the dorsal skin of the mouse back intradermally. Nevertheless, this procedure requires shaving and dehairing of the skin, which also induces irritation and does not guarantee steady-state conditions prior to inoculation.
For these reasons, we decided to perform the procedure as described in the protocol.
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