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This protocol is very simple to reproduce. I isolated only the...

Pia Giovannelli
Pia Giovannelli Nov 30, 2018
protocol Protocol: Subcellular Fractionation of Cultured Human Cell Lines
This protocol is very simple to reproduce. I isolated only the nuclear and cytoplasmic fractions, not membrane. I added two more washes with SF buffer before usinf the NL buffer because into the nuclear fraction I had too much tubulin.
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