Improve Research Reproducibility A Bio-protocol resource

HiThanks for the detailed protocol. Actually I have to get T...

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deepak sumbria Nov 26, 2018
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Hi
Thanks for the detailed protocol. Actually I have to get T cell in in-vitro culture. But i am confused how to make 5 μg/μl of anti-CD3. I have also seen Biolegend, catalog number:100302, but the concentration was 0.5 mg/ml, so i am confused how to make 5 μg/μl. Can you help me in t Read more Read less more less
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deepak sumbria Answered Nov 28, 2018

GADVASU

Thanks Jia Li for valuable support
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Jia Li Author Answered Nov 28, 2018

SRI International

Hi，it‘s 5ug/ml. Sorry for the typing mistake
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