If you start from a large amount of material it might be better...

Antoine Danon Nov 19, 2018

Protocol: Protoplast Preparation and Determination of Cell Death

If you start from a large amount of material it might be better to use a 50 mL tube for the centrifugation to increase the surface and separate better the dead from the living protoplasts. You can also increase slightly the centrifugation time, if the cushion is not clean as on figure 1 an Read more Read less

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