Dear authors,I have been trying to reproduce your protocol,...

William Jules Nicolas Nov 19, 2018

Protocol: Protoplast Preparation and Determination of Cell Death

Dear authors,

I have been trying to reproduce your protocol, which works. However I feel that even after centrifugation in sucrose and isolation of the cushion, I still have a considerable amount of dead cells when I screen the fraction under light microscopy.

I would Read more Read less

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