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Hi, I tried this protocol hoping to extract nuclei, thus I only...

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Wen Haoyu Nov 4, 2018
protocol Protocol: NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells
Hi, I tried this protocol hoping to extract nuclei, thus I only completed the NP-40 fractionation part using 293T cells, after which I did western blot with Anti-Actin antibody produced in mouse and Anti-Lamin B antibody produced in rabbit. But the reseult shows that the nuclei part still Read more Read less more less
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