I am planning to perform SDD-AGE to detect amyloids for a protein...

JS
Jagmohan Singh
Oct 16, 2018
protocol Protocol: Semi-denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE)
I am planning to perform SDD-AGE to detect amyloids for a protein with molecular mass of >160kD. Would appreciate suggestions like percentage of agarose gel, length of run and voltage and conditions for blotting and detection. Thanks
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