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Hello, I checked from Addgene website and there is no item #90095...
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Nisa Mohammed Oct 3, 2018
Hello,
I checked from Addgene website and there is no item #90095 and #90096 as you claimed. I am trying to purify from #90094. Can I use your protocol for this?
Nisa
I checked from Addgene website and there is no item #90095 and #90096 as you claimed. I am trying to purify from #90094. Can I use your protocol for this?
Nisa
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Daan C. Swarts Author Answered Jun 17, 2019
Department of Biochemistry, University of Zurich, Switzerland
Dear Nisa,
I am curious if you were successful with the purification! Do you need any other input?
With kind regards,
Daan
I am curious if you were successful with the purification! Do you need any other input?
With kind regards,
Daan
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0 comments ShareDaan C. Swarts Author Answered Oct 4, 2018
Department of Biochemistry, University of Zurich, Switzerland
Dear Nisa,
To add to the comment of Prarthana: I think indeed it should be possible to express and purify FnCas12a from #90094 with the same protocol. However, we never tested this expression plasmid ourselves, so we don't know what the yield will be. Maybe it will be informative for other readers/users of the protocol if you can share if you succeeded with the purification and what the yield was!
With kind regards,
Daan
To add to the comment of Prarthana: I think indeed it should be possible to express and purify FnCas12a from #90094 with the same protocol. However, we never tested this expression plasmid ourselves, so we don't know what the yield will be. Maybe it will be informative for other readers/users of the protocol if you can share if you succeeded with the purification and what the yield was!
With kind regards,
Daan
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0 comments ShareNisa Mohammed Answered Oct 3, 2018
King Abdullah University
Thank you so much for your reply!
Cheers,
Cheers,
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0 comments ShareP Mohanraju Author Answered Oct 3, 2018
Wageningen University and Research
Dear Nisa,
These are the addgene links for the plasmids #90095 and #90096 : https://www.addgene.org/90095/ & https://www.addgene.org/90096/.
You can also use this protocol to purify hFnCpf1 from the addgene #90094 plasmid.
All the best!
These are the addgene links for the plasmids #90095 and #90096 : https://www.addgene.org/90095/ & https://www.addgene.org/90096/.
You can also use this protocol to purify hFnCpf1 from the addgene #90094 plasmid.
All the best!
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