Number of cells obtained were too low.

protocol Protocol: Isolation and Culture of Peritoneal Cell-derived Mast Cells
Number of cells obtained were too low.
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Krisztina V. Vukman Author Answered Oct 1, 2018

Dublin City University

Cell number is depend on many things:
- From old mice, you can get more cells
- concentration and source of IL-3 is important as well
- During peritoneal lavage the "massage" step is very important
- Cells are quite sensitive. They do not like harsh pipetting, changes in temperature or pH, long centrifugation, etc.
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