Hi,I have a general question about polysome profiling. I used...

MT
Mel Todd
Sep 11, 2018
protocol Protocol: Polysome Fractionation to Analyze mRNA Distribution Profiles
Hi,
I have a general question about polysome profiling. I used another protocol where the lysis buffer is (20 mM Tris (pH 7.4), 150 mM NaCl, 5 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 1% sodium deoxycholate, 50 µg/ml cycloheximide, complete protease inhibitor cocktail (1 final concentrati
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