Hello Mr. Burby,sorry for the late response. I switched to pPB41...

protocol Protocol: CRISPR/Cas9 Editing of the Bacillus subtilis Genome
Hello Mr. Burby,
sorry for the late response. I switched to pPB41 for Spacer cloning procedure. The first attempt to incorporate the Spacer seemed to be successful after gel electrophoresis, but the sequencing results said it´s not incorporated. Also the first Spacer and the second CR
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