How long would the OMVs be stable at -80 °C vs. 4°C? and what...

TT
Tuan Minh Tran
Aug 19, 2018
protocol Protocol: Preparation of Outer Membrane Vesicle from Escherichia coli
How long would the OMVs be stable at -80 °C vs. 4°C? and what is the best way to test for OMV integrity you would recommend (TEM? protein concentration?). Thank you.
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Oh Youn Kim Author Answered Sep 17, 2018

Life Sciences Department, Pohang University of Science and Technology (POSTECH), Republic of Korea

According to our previous data on the stability of OMVs in different conditions, both -80 and 4 C showed similar stability. But I would recommend -80 C for long storage. To check the OMV integrity, I recommend analyzing the size of OMV and shape using dynamic light scattering or TEM imaging.
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