Good afternoon!I tried to seed human microvascular endothelium...

JW
Jie Wang
Aug 6, 2018
protocol Protocol: In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
Good afternoon!
I tried to seed human microvascular endothelium cells in the chamber slide. However, I could not remove the pre-filled liquid in the chamber continuously so that I could not be sure the volume I removed.
Another question is which kind of microscope I need to use
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Takemi Tanaka Author Answered Aug 13, 2018

Stephenson Cancer Center, University of Oklahoma Health Sciences Center, USA

You will remove volume that you add to the chamber. Say add 100uL, and the same volume will be removed from another port. Just be careful not to dry channel, it is difficult to fill with fluid if you dry. Any sort of inverted microscope can be used and we typically use X20 magnification. Hope this clarifies your question.
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