Hi. I usually use total RNA as sort of positive control for...

Protocol: Dot Blot Analysis of N⁶-methyladenosine RNA Modification Levels

Hi. I usually use total RNA as sort of positive control for a strong signal, in fact as said m6A is found in any RNA species. So, when I isolate mRNA (Dynabeads for polyA), the dot is less intense compared to the total RNA "control". I have extracted RNA with either Trizol or QUIAGEN/Norge Read more Read less

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