Thank you for the protocol. I am having trouble detecting YAP...

Protocol: Non-radioactive LATS in vitro Kinase Assay

Thank you for the protocol. I am having trouble detecting YAP after bacterial lysis. IPTG seems to induce a whole series of bands, and nothing obvious at full length YAP size, but the IPTG does have effect.

First, is the plasmid full length YAP? In the citation of the source th Read more Read less

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