Does anyone know the optimal cell concentration or cell number...

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Xiaogang Zhang
Jul 31, 2018
protocol Protocol: In vitro Culture of Human PBMCs
Does anyone know the optimal cell concentration or cell number of PBMCs cultured in 96 wells?
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Balachandran Ravindran Author Answered Jul 31, 2018

Infectious Disease Biology, Institute of Life Sciences, India

We usually take PBMC concentration of 1 million cells (a mixture of Monocytes and lymphocytes) and seed 100 ul per well of this suspension in our cultures
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