Hi,I've read the reference-paper and have a question about...

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xiao
Jul 31, 2018
protocol Protocol: Purification of His-ubiquitin Proteins from Mammalian Cells
Hi,I've read the reference-paper and have a question about 6his-ubiquitin plasmid. A number of genes can code ubiquitin unit, for example UBB and UBC. Which gene were used for the construction of plasmid ? or just 76 amino acids for one unit of ubiquitin
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xiao Answered Aug 15, 2018

Sun Yat-Sen University

Thank you, Vansesa
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Vanesa Olivares-Illana Author Answered Jul 31, 2018

Instituto de Física, Universidad Autónoma de San Luis Potosí

Hello Xiao

Indeed we used just one 76 aa coding gen, and the cell add an unknown number of ubiquitin His tagget to the protein. I hope this information helps to answer your question.

Vanesa
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