Hi,I've read the reference-paper and have a question about 6his-ubiquitin plasmid. A number of genes can code ubiquitin unit, for example UBB and UBC. Which gene were used for the construction of plasmid ? or just 76 amino acids for one unit of ubiquitin
Instituto de Física, Universidad Autónoma de San Luis Potosí
Hello Xiao
Indeed we used just one 76 aa coding gen, and the cell add an unknown number of ubiquitin His tagget to the protein. I hope this information helps to answer your question.