Improve Research Reproducibility A Bio-protocol resource

Hi Brian, I used DMSO to dilute DiOC6, because DiOC6 insoluble...

cs
chuan shen Jul 29, 2018
protocol Protocol: Isolation and Quantification of Plant Extracellular Vesicles
Hi Brian,
I used DMSO to dilute DiOC6, because DiOC6 insoluble in Tris-Hcl, even though heating.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Brian D. Rutter Author Answered Jul 29, 2018

Indiana University

That’s correct. The 10 mM stock of DiOC6 should be dissolved in DMSO. Then, dilute it in Tris-HCl to make a 100 uM solution.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer